Calcium and Silicon Precipitation in Pinus taeda Needles in Response to Soil Application of Cellulosic Residue
Julierme Zimmer Barbosa, Evelyn Joslin Mendes, Shizuo Maeda, Anne Luiz Sass, Eloa Araujo, Ederlan Magri, Stephen Arthur Prior, Antonio Carlos Vargas Motta
http://dx.doi.org/10.1590/2179-8087-FLORAM-2024-0048
FLORAM, vol.32, n3, e20240048, 2025
Abstract
The use of biosolids as a sustainable alternative in the management of planted Pinus taeda forests affects availability of nutrients and beneficial elements. Calcium (Ca) and Silicon (Si) play important roles in plant regulatory and protective systems; therefore, our objective was to use scanning electron microscopy combined with energy-dispersive spectroscopy (SEM-EDS) to analyze the accumulation of elements in P. taeda needles grown in soil that received 0 to 60 Mg ha-1 applications of cellulosic waste. Microanalyses of needle sections were performed using a SEM with elemental detection by EDS. Mapping mode allowed for detection and analysis of Ca, Si, and C distributions in needle sections. Calcium and Si precipitation occurred in needles, with Ca accumulating in the phloem and Si in the epidermal cells. Application of 60 Mg ha-1 changed the availability and accumulation of elements, which resulted in more Ca crystals and fewer Si crystals.
Keywords
Calcium oxalate; phytoliths; electron microscopy
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Submitted date:
11/28/2024
Accepted date:
08/02/2025
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